Mouse BMP-2 (Bone Morphogenetic Protein 2) CLIA Kit
The Mouse BMP-2 (Bone Morphogenetic Protein 2) CLIA Kit is specifically designed for the accurate measurement of BMP-2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.BMP-2 is a key protein involved in bone formation and regeneration, making it an essential factor in skeletal development and repair.
Studies have also shown its role in cell differentiation, organogenesis, and tissue homeostasis. With its importance in various biological processes, the Mouse BMP-2 CLIA Kit serves as a valuable tool for investigating bone-related diseases, tissue engineering, and other biomedical research areas.Order now and discover the potential of BMP-2 in your research projects with the Mouse BMP-2 CLIA Kit from Assay Genie.
Product Name:
Mouse BMP-2 (Bone Morphogenetic Protein 2) CLIA Kit
SKU:
MOES00122
Target:
Mouse BMP-2 (Bone Morphogenetic Protein 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse BMP-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BMP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse BMP-2. You can calculate the concentration of Mouse BMP-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-104
96-112
99-114
Average (%)
95
103
105
1:4
Range (%)
88-102
87-101
89-101
Average (%)
96
92
96
1:8
Range (%)
96-109
99-112
100-113
Average (%)
102
105
106
1:16
Range (%)
94-109
86-99
89-103
Average (%)
102
93
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
98
EDTA plasma (n=5)
87-100
92
Cell culture media (n=5)
84-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
55.21
135.89
340.78
56.78
130.55
355.14
Standard deviation
5.1
11.39
31.32
7.18
9.9
37.68
C V (%)
9.24
8.38
9.19
12.65
7.58
10.61
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse BMP-2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse BMP-2 in samples. No significant cross-reactivity or interference between Mouse BMP-2 and analogues was observed.
