Mouse BMP-4 (Bone Morphogenetic Protein 4) CLIA Kit
The Mouse BMP-4 (Bone Morphogenetic Protein 4) CLIA Kit is a reliable and accurate tool for detecting BMP-4 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.BMP-4 is a key protein involved in bone formation and development, as well as in various other cellular processes.
It plays a critical role in regulating cell differentiation and proliferation, making it a valuable biomarker for studying skeletal disorders, developmental defects, and other related conditions.With the Mouse BMP-4 CLIA Kit, researchers can confidently explore the role of BMP-4 in diverse biological processes and diseases, paving the way for new insights and potential therapeutic interventions.
Product Name:
Mouse BMP-4 (Bone Morphogenetic Protein 4) CLIA Kit
SKU:
MOES00123
Target:
Mouse BMP-4 (Bone Morphogenetic Protein 4)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse BMP-4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BMP-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BMP-4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse BMP-4. You can calculate the concentration of Mouse BMP-4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-115
101-114
91-102
Average (%)
105
108
96
1:4
Range (%)
101-113
92-104
103-117
Average (%)
107
98
110
1:8
Range (%)
96-106
90-102
100-116
Average (%)
101
96
108
1:16
Range (%)
88-104
88-104
87-101
Average (%)
95
95
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
96
EDTA plasma (n=5)
100-118
107
Cell culture media (n=5)
93-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
43.6
71.55
312.63
47.83
78.44
304.45
Standard deviation
4.76
7.89
32.45
5.81
8.98
26.91
C V (%)
10.92
11.03
10.38
12.15
11.45
8.84
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse BMP-4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse BMP-4 in samples. No significant cross-reactivity or interference between Mouse BMP-4 and analogues was observed.
