Mouse BMP-6 (Bone Morphogenetic Protein 6) CLIA Kit
The Mouse BMP-6 (Bone Morphogenetic Protein 6) CLIA Kit is a specialized assay designed for the accurate measurement of BMP-6 levels in mouse samples including serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures precise and reproducible results, making it an invaluable tool for various research applications.BMP-6 is a critical signaling molecule involved in the regulation of bone and cartilage development, as well as in the maintenance and repair of various tissues in the body. Dysregulation of BMP-6 has been associated with a range of diseases and conditions, including skeletal disorders, metabolic diseases, and cancer.
By detecting and quantifying BMP-6 levels, researchers can gain insight into its role in these conditions and explore potential therapeutic interventions.Overall, the Mouse BMP-6 CLIA Kit offers researchers a reliable and efficient method for studying the role of BMP-6 in physiological and pathological processes, providing valuable data for further research and drug development efforts.
Product Name:
Mouse BMP-6 (Bone Morphogenetic Protein 6) CLIA Kit
SKU:
MOES00124
Target:
Mouse BMP-6 (Bone Morphogenetic Protein 6)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse BMP-6. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BMP-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BMP-6, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse BMP-6. You can calculate the concentration of Mouse BMP-6 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-102
85-97
87-99
Average (%)
96
91
94
1:4
Range (%)
92-109
92-106
88-104
Average (%)
99
97
95
1:8
Range (%)
96-108
91-105
91-102
Average (%)
102
98
96
1:16
Range (%)
93-108
98-109
83-96
Average (%)
101
104
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-110
102
EDTA plasma (n=5)
92-109
99
Cell culture media (n=5)
92-102
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.49
89.9
406.98
54.92
92.25
389.8
Standard deviation
6.45
7.82
33.78
5.14
11.04
35.67
C V (%)
11.84
8.7
8.3
9.36
11.97
9.15
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse BMP-6 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse BMP-6 in samples. No significant cross-reactivity or interference between Mouse BMP-6 and analogues was observed.
