Mouse BMP-6 (Bone Morphogenetic Protein 6) ELISA Kit
The Mouse BMP-6 (Bone Morphogenetic Protein 6) ELISA Kit is meticulously developed for the precise and quantitative analysis of BMP-6 levels in a variety of mouse biological samples.
BMP-6, a member of the bone morphogenetic protein family, plays a crucial role in regulating cell growth, differentiation, and development, making it essential for various biological processes. Our Mouse BMP-6 ELISA Kit ensures exceptional sensitivity and specificity, providing accurate measurements of BMP-6 concentrations. With stringent quality control measures, this kit offers robust performance and reproducible results, enabling researchers to unravel the intricate functions of BMP-6 in physiological and pathological conditions. The user-friendly design of this kit makes it an excellent choice for researchers seeking reliable and accurate data for their investigations.
Product Name:
Mouse BMP-6 (Bone Morphogenetic Protein 6) ELISA Kit
SKU:
AEES00214
Target:
Mouse BMP-6 (Bone Morphogenetic Protein 6)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse BMP-6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BMP-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BMP-6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse BMP-6. You can calculate the concentration of Mouse BMP-6 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
92-103
99-106
Average (%)
98
98
103
1:4
Range (%)
100-110
93-108
95-105
Average (%)
104
100
102
1:8
Range (%)
92-105
89-100
98-109
Average (%)
98
97
105
1:16
Range (%)
83-98
99-110
92-105
Average (%)
91
103
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-108
102
EDTA plasma (n=5)
90-103
97
Cell culture media (n=5)
85-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.08
106.48
432.89
43.47
100.29
426.36
Standard deviation
2.74
5.0
22.64
2.59
4.65
22.51
C V (%)
5.82
4.7
5.23
5.96
4.64
5.28
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse BMP-6 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse BMP-6 in samples. No significant cross-reactivity or interference between Mouse BMP-6 and analogues was observed.
