Mouse BMP-7 (Bone Morphogenetic Protein 7) CLIA Kit
The Mouse BMP-7 (Bone Morphogenetic Protein-7) CLIA Kit is specifically designed for the accurate quantification of BMP-7 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.BMP-7 is a vital protein that plays a key role in bone development and regeneration, as well as in the regulation of cell growth and differentiation. Dysregulation of BMP-7 has been linked to various diseases such as bone disorders, kidney diseases, and cancer, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
With its advanced technology and easy-to-follow protocol, the Mouse BMP-7 CLIA Kit from Assaygenie provides researchers with a reliable tool for studying the function and mechanisms of BMP-7 in mouse models, ultimately advancing our understanding of various physiological and pathological processes.
Product Name:
Mouse BMP-7 (Bone Morphogenetic Protein 7) CLIA Kit
SKU:
MOES00125
Target:
Mouse BMP-7 (Bone Morphogenetic Protein 7)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse BMP-7. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BMP-7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BMP-7, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse BMP-7. You can calculate the concentration of Mouse BMP-7 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-120
96-108
102-116
Average (%)
109
102
110
1:4
Range (%)
87-100
100-115
94-108
Average (%)
94
109
102
1:8
Range (%)
90-102
95-110
85-99
Average (%)
95
101
91
1:16
Range (%)
83-96
95-108
100-116
Average (%)
90
103
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-102
93
EDTA plasma (n=5)
84-99
91
Cell culture media (n=5)
92-104
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.42
68.75
194.3
25.66
70.65
182.73
Standard deviation
2.2
4.87
21.1
2.26
6.97
18.15
C V (%)
9.39
7.08
10.86
8.81
9.87
9.93
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse BMP-7 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse BMP-7 in samples. No significant cross-reactivity or interference between Mouse BMP-7 and analogues was observed.
