The Mouse C-P-C Peptide CLIA Kit is a highly sensitive and specific assay designed for the precise measurement of mouse C-P-C peptide levels in biological samples such as serum, plasma, and tissue homogenates. This kit provides accurate and reproducible results, making it an ideal tool for researchers studying the role of C-P-C peptide in various physiological processes.C-P-C peptide is a key regulator of glucose metabolism and insulin sensitivity, playing a crucial role in the pathogenesis of diabetes and metabolic disorders.
By measuring C-P-C peptide levels, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify new targets for therapeutic intervention.With its user-friendly protocol and reliable performance, the Mouse C-P-C Peptide CLIA Kit offers a convenient solution for analyzing C-P-C peptide levels in mouse models, facilitating groundbreaking research in diabetes, obesity, and related fields.
Product Name:
Mouse C-P (C-Peptide) CLIA Kit
SKU:
MOES00200
Target:
Mouse C-P (C-Peptide)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse C-P. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse C-P and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse C-P, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse C-P. You can calculate the concentration of Mouse C-P in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
87-101
95-108
Average (%)
101
93
101
1:4
Range (%)
96-107
94-108
87-102
Average (%)
101
101
93
1:8
Range (%)
86-101
87-100
94-111
Average (%)
93
92
102
1:16
Range (%)
89-105
97-112
99-111
Average (%)
97
104
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
97
EDTA plasma (n=5)
93-105
99
Cell culture media (n=5)
88-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
93.46
276.82
787.41
86.95
253.12
821.88
Standard deviation
9.49
26.74
47.56
8.07
17.95
90.32
C V (%)
10.15
9.66
6.04
9.28
7.09
10.99
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse C-P concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse C-P in samples. No significant cross-reactivity or interference between Mouse C-P and analogues was observed.
