The Mouse C4a (Complement Component 4a) ELISA Kit is a reliable tool for detecting levels of C4a in mouse serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides accurate and reproducible results, making it suitable for a variety of research applications.C4a is an essential component of the complement system, playing a key role in immune response and inflammation. Abnormal levels of C4a have been associated with various diseases, including autoimmune disorders, infectious diseases, and inflammatory conditions.
Therefore, monitoring C4a levels can provide valuable insights into the pathogenesis of these diseases and aid in the development of potential therapeutic interventions.Overall, the Mouse C4a ELISA Kit offers researchers a reliable and efficient way to study the role of C4a in mouse models, advancing our understanding of immune regulation and inflammatory processes.
Product Name:
Mouse C4a (Complement Component 4a) ELISA Kit
SKU:
MOES00901
Target:
Mouse C4a (Complement Component 4a)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse C4a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse C4a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse C4a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse C4a. You can calculate the concentration of Mouse C4a in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-111
84-97
92-107
Average (%)
102
91
98
1:4
Range (%)
92-105
84-96
87-100
Average (%)
99
89
92
1:8
Range (%)
88-101
78-92
82-96
Average (%)
94
85
89
1:16
Range (%)
90-105
84-96
87-98
Average (%)
96
90
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
101
EDTA plasma (n=5)
87-100
92
Cell culture media (n=5)
96-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
247.32
511.51
2112.16
245.3
535.88
2299.95
Standard deviation
16.62
21.79
87.87
16.36
26.2
99.59
C V (%)
6.72
4.26
4.16
6.67
4.89
4.33
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse C4a concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse C4a in samples. No significant cross-reactivity or interference between Mouse C4a and analogues was observed.
