The Mouse CA9 (Carbonic Anhydrase IX) CLIA Kit is a cutting-edge assay designed for the precise measurement of CA9 levels in mouse samples. This kit offers superior sensitivity and specificity, guaranteeing accurate and consistent results for various experimental applications.Carbonic Anhydrase IX is an important enzyme involved in regulating pH balance and promoting cell survival in hypoxic environments.
Its expression is elevated in various cancers, making it a valuable biomarker for cancer research and drug development.With the Mouse CA9 CLIA Kit, researchers can easily quantify CA9 levels in mouse serum, plasma, and tissue homogenates, providing valuable insights into the role of this enzyme in disease progression and potential therapeutic targets.
Product Name:
Mouse CA9 (Carbonic Anhydrase IX) CLIA Kit
SKU:
MOES00141
Target:
Mouse CA9 (Carbonic Anhydrase IX)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CA9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CA9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CA9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CA9. You can calculate the concentration of Mouse CA9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
83-95
102-120
Average (%)
106
90
110
1:4
Range (%)
89-101
104-121
91-103
Average (%)
95
110
97
1:8
Range (%)
88-101
100-116
102-116
Average (%)
94
108
109
1:16
Range (%)
84-97
90-104
97-112
Average (%)
90
95
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
95
EDTA plasma (n=5)
86-100
92
Cell culture media (n=5)
93-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.37
67.6
169.7
26.8
73.41
155.12
Standard deviation
3.07
7.83
14.95
2.53
8.43
12.04
C V (%)
12.1
11.58
8.81
9.44
11.48
7.76
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CA9 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CA9 in samples. No significant cross-reactivity or interference between Mouse CA9 and analogues was observed.
