The Mouse Carbonic Anhydrase I (CA1) ELISA Kit is specifically designed for the accurate and precise measurement of mouse carbonic anhydrase I levels in serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Carbonic anhydrase I is an important enzyme involved in the regulation of acid-base balance and the production of bicarbonate ions. It has been implicated in various physiological and pathological processes, including bone resorption, kidney function, and acid-base disorders.
Monitoring carbonic anhydrase I levels can provide valuable insights into these processes and facilitate the development of potential therapeutic interventions.With its cutting-edge technology and superior performance, the Mouse Carbonic Anhydrase I (CA1) ELISA Kit is an essential tool for researchers studying carbonic anhydrase I's role in health and disease. Its versatility and reliability make it a valuable asset for advancing scientific knowledge and ultimately improving human health.
Product Name:
Mouse Carbonic Anhydrase I (CA1) ELISA Kit
Product Code:
MODL00188
Size:
96 Assays
Target:
CA1
Synonyms:
CA-I, CAI, Car1, CAB, Carbonic anhydrase B, Carbonate dehydratase I
Detection Method:
Sandwich
Range:
0.781-50IU/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
