The Mouse Casp7 (Caspase-7) CLIA Kit is specifically designed for the precise measurement of Caspase-7 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.Caspase-7 is an important protein involved in apoptotic pathways, playing a key role in programmed cell death. Dysregulation of Caspase-7 has been implicated in various diseases, including cancer, autoimmune disorders, and neurodegenerative conditions.
Therefore, reliable detection of Caspase-7 levels is critical for understanding these diseases and developing potential therapeutic interventions.The Mouse Casp7 CLIA Kit from Assay Genie provides researchers with a valuable tool for studying the role of Caspase-7 in disease pathogenesis and evaluating the efficacy of novel treatment strategies. Trust in the accuracy and precision of this kit to advance your research and contribute to the advancement of scientific knowledge in the field of apoptosis and cell death mechanisms.
Product Name:
Mouse CASP7 (Caspase 7) CLIA Kit
SKU:
MOES00152
Target:
Mouse CASP7 (Caspase 7)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CASP7. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CASP7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CASP7, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CASP7. You can calculate the concentration of Mouse CASP7 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-114
93-105
87-100
Average (%)
106
98
94
1:4
Range (%)
94-108
95-109
91-103
Average (%)
101
100
97
1:8
Range (%)
89-101
87-100
91-103
Average (%)
96
94
96
1:16
Range (%)
99-113
88-102
92-107
Average (%)
107
96
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-114
104
EDTA plasma (n=5)
96-108
102
Cell culture media (n=5)
95-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.24
241.74
978.71
90.55
219.19
1004.34
Standard deviation
9.07
28.26
65.18
8.19
23.83
89.89
C V (%)
9.94
11.69
6.66
9.04
10.87
8.95
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CASP7 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CASP7 in samples. No significant cross-reactivity or interference between Mouse CASP7 and analogues was observed.
