The Mouse Casp8 (Caspase-8) CLIA Kit is specifically designed for the quantitative detection of caspase-8 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Caspase-8 is a key enzyme involved in apoptosis, playing a critical role in programmed cell death. Dysregulation of caspase-8 has been implicated in various diseases, including cancer, autoimmune disorders, and neurodegenerative conditions.
As such, measuring caspase-8 levels can provide valuable insights into disease mechanisms and potential therapeutic targets.With its high performance and user-friendly protocol, the Mouse Casp8 CLIA Kit is an essential tool for researchers studying apoptosis, cell death pathways, and related diseases in mouse models. Trust assaygenie.com to provide reliable and innovative solutions for your research needs.
Product Name:
Mouse CASP8 (Caspase 8) CLIA Kit
SKU:
MOES00054
Target:
Mouse CASP8 (Caspase 8)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CASP8. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CASP8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CASP8, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CASP8. You can calculate the concentration of Mouse CASP8 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
85-97
90-104
Average (%)
99
91
97
1:4
Range (%)
85-96
95-111
89-101
Average (%)
90
102
95
1:8
Range (%)
97-111
86-99
99-115
Average (%)
105
92
106
1:16
Range (%)
99-113
86-98
86-98
Average (%)
106
91
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-115
105
EDTA plasma (n=5)
101-114
107
Cell culture media (n=5)
98-113
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
109.05
309.64
857.47
105.13
317.96
896.92
Standard deviation
11.71
31.12
91.58
11.41
37.33
67.09
C V (%)
10.74
10.05
10.68
10.85
11.74
7.48
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CASP8 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CASP8 in samples. No significant cross-reactivity or interference between Mouse CASP8 and analogues was observed.
