The Mouse CASP9 (Caspase 9) CLIA Kit is a highly reliable and sensitive assay designed for the accurate measurement of caspase-9 levels in mouse samples. This kit is suitable for use with serum, plasma, and cell culture supernatants, providing researchers with a valuable tool for studying apoptosis and cell death mechanisms in a variety of research applications.Caspase-9 is a key mediator of the intrinsic apoptotic pathway and is involved in regulating cell death in response to various stimuli.
Dysregulation of caspase-9 activity has been implicated in numerous diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases. The Mouse CASP9 CLIA Kit offers researchers a precise and efficient method for investigating the role of caspase-9 in these conditions and developing potential therapeutic interventions.
Product Name:
Mouse CASP9 (Caspase 9) CLIA Kit
SKU:
MOES00153
Target:
Mouse CASP9 (Caspase 9)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CASP9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CASP9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CASP9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CASP9. You can calculate the concentration of Mouse CASP9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
88-104
99-115
Average (%)
104
95
106
1:4
Range (%)
98-115
99-111
86-100
Average (%)
106
105
93
1:8
Range (%)
85-99
103-115
84-97
Average (%)
91
109
91
1:16
Range (%)
90-102
91-101
94-111
Average (%)
95
96
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-109
103
EDTA plasma (n=5)
99-111
105
Cell culture media (n=5)
90-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
102.09
202.89
737.61
92.72
184.35
727.15
Standard deviation
10.21
21.32
78.78
7.53
19.87
62.9
C V (%)
10.0
10.51
10.68
8.12
10.78
8.65
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CASP9 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CASP9 in samples. No significant cross-reactivity or interference between Mouse CASP9 and analogues was observed.
