The Mouse Cationic Amino Acid Transporter-1 (CAT-1) ELISA Kit is specifically designed for the accurate detection of CAT-1 levels in mouse serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring precise and reliable results that are essential for a wide range of research applications.CAT-1 is an important protein involved in the transport of cationic amino acids across cell membranes, playing a crucial role in various physiological processes such as protein synthesis, cell signaling, and immune response. Dysregulation of CAT-1 has been linked to various diseases including metabolic disorders, neurological disorders, and cancer, making it a valuable biomarker for studying these conditions and developing potential therapeutic strategies.
With its high-quality components and optimized protocols, the Mouse CAT-1 ELISA Kit offers researchers a powerful tool to accurately quantify CAT-1 levels in biological samples, providing valuable insights into the molecular mechanisms underlying disease pathogenesis and progression. Order now and enhance your research capabilities with this cutting-edge ELISA kit from Assay Genie.
SLC7A1, HCAT1, ATRC1, ERR, Solute Carrier Family 7 Member 1,Cationic Amino Acid Transporter,y+, Ecotropic Retroviral Receptor, High Affinity Cationic Amino Acid Transporter 1
Detection Method:
Sandwich
Range:
0.312-20ng/ml
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
