The Mouse CCL1 (Chemokine C-C Motif Ligand 1) CLIA Kit is a reliable and accurate tool for measuring levels of CCL1 in mouse serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.CCL1 is a chemokine involved in the regulation of immune responses and cell migration, playing a critical role in inflammatory processes and immune system function.
Dysregulation of CCL1 has been linked to various disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Mouse CCL1 CLIA Kit is a valuable asset for researchers looking to investigate the role of CCL1 in immunology, inflammation, and disease pathways in mouse models.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CCL1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CCL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CCL1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CCL1. You can calculate the concentration of Mouse CCL1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
91-105
86-100
Average (%)
99
96
92
1:4
Range (%)
97-108
97-113
90-103
Average (%)
103
104
95
1:8
Range (%)
89-103
96-111
90-103
Average (%)
95
103
97
1:16
Range (%)
91-106
85-99
88-102
Average (%)
97
90
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
99
EDTA plasma (n=5)
101-116
107
Cell culture media (n=5)
89-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.89
192.52
722.97
104.18
195.55
687.16
Standard deviation
9.1
15.25
79.45
8.72
16.0
49.82
C V (%)
9.2
7.92
10.99
8.37
8.18
7.25
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CCL1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CCL1 in samples. No significant cross-reactivity or interference between Mouse CCL1 and analogues was observed.
