Mouse CCL3L1 (Chemokine C-C-Motif Ligand 3 Like Protein 1) CLIA Kit
The Mouse CCL3L1 (Chemokine C-C Motif Ligand 3-Like Protein 1) CLIA Kit is a versatile and reliable assay designed for the accurate detection of CCL3L1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.CCL3L1 is a chemokine protein involved in inflammation, immune response, and cell migration.
Its role in regulating the immune system makes it a crucial biomarker for studying immune-related diseases, such as autoimmune disorders, infectious diseases, and inflammatory conditions. The Mouse CCL3L1 CLIA Kit provides researchers with a valuable tool for investigating the role of CCL3L1 in various biological processes and identifying potential therapeutic targets.
Product Name:
Mouse CCL3L1 (Chemokine C-C-Motif Ligand 3 Like Protein 1) CLIA Kit
SKU:
MOES00164
Target:
Mouse CCL3L1 (Chemokine C-C-Motif Ligand 3 Like Protein 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CCL3L1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CCL3L1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CCL3L1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CCL3L1. You can calculate the concentration of Mouse CCL3L1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
98-113
95-110
Average (%)
97
104
101
1:4
Range (%)
91-105
89-99
93-107
Average (%)
99
94
101
1:8
Range (%)
86-98
99-112
91-103
Average (%)
93
107
98
1:16
Range (%)
97-111
99-113
88-100
Average (%)
103
105
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
96
EDTA plasma (n=5)
92-105
97
Cell culture media (n=5)
98-115
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.8
156.75
408.57
51.72
155.9
372.47
Standard deviation
4.3
17.23
28.72
6.27
17.74
31.29
C V (%)
8.3
10.99
7.03
12.12
11.38
8.4
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CCL3L1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CCL3L1 in samples. No significant cross-reactivity or interference between Mouse CCL3L1 and analogues was observed.
