The Mouse CCND1 (Cyclin D1) CLIA Kit is a highly sensitive and specific assay designed for the quantitative measurement of Cyclin D1 levels in mouse samples. This kit enables accurate detection of Cyclin D1 in serum, plasma, and tissue lysates, providing reliable and reproducible results for a variety of research applications.Cyclin D1 is a key regulator of the cell cycle, controlling cell proliferation and differentiation.
Dysregulation of Cyclin D1 has been implicated in various diseases, including cancer, making it an important biomarker for understanding disease mechanisms and developing targeted therapies.The Mouse CCND1 (Cyclin D1) CLIA Kit offers researchers a reliable tool for studying the role of Cyclin D1 in mouse models of disease, providing valuable insights into disease pathogenesis and potential therapeutic interventions.
Product Name:
Mouse CCND1 (Cyclin-D1) CLIA Kit
SKU:
MOES00212
Target:
Mouse CCND1 (Cyclin-D1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CCND1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CCND1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CCND1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CCND1. You can calculate the concentration of Mouse CCND1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
100-117
95-108
Average (%)
92
108
103
1:4
Range (%)
96-111
93-104
99-115
Average (%)
104
99
107
1:8
Range (%)
90-105
89-99
92-104
Average (%)
97
94
98
1:16
Range (%)
91-106
99-114
101-119
Average (%)
98
106
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
101
EDTA plasma (n=5)
96-109
102
Cell culture media (n=5)
91-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
101.08
200.99
916.22
97.14
203.15
853.87
Standard deviation
12.66
21.16
76.78
8.45
19.06
55.07
C V (%)
12.52
10.53
8.38
8.7
9.38
6.45
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CCND1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CCND1 in samples. No significant cross-reactivity or interference between Mouse CCND1 and analogues was observed.
