Mouse CCRL1 (Chemokine C-C-Motif Receptor Like Protein 1) CLIA Kit
The Mouse CCRL1 (Chemokine C-C motif receptor-like protein 1) CLIA Kit is a powerful tool for the quantitative measurement of CCRL1 levels in mouse samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of scientific investigations.CCRL1 is a key protein involved in immune responses, inflammation, and cell migration. It plays a critical role in regulating the activation and recruitment of immune cells to sites of inflammation, making it a valuable target for studying inflammatory diseases, autoimmune disorders, and cancer progression.
With its reliable performance and wide range of applications, the Mouse CCRL1 CLIA Kit is an essential resource for researchers seeking to explore the role of CCRL1 in health and disease, paving the way for potential new therapeutic interventions.
Product Name:
Mouse CCRL1 (Chemokine C-C-Motif Receptor Like Protein 1) CLIA Kit
SKU:
MOES00165
Target:
Mouse CCRL1 (Chemokine C-C-Motif Receptor Like Protein 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CCRL1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CCRL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CCRL1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CCRL1. You can calculate the concentration of Mouse CCRL1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-98
95-113
89-99
Average (%)
91
103
94
1:4
Range (%)
92-105
99-111
89-102
Average (%)
97
105
94
1:8
Range (%)
99-111
95-105
85-97
Average (%)
105
100
91
1:16
Range (%)
93-108
104-120
88-102
Average (%)
99
110
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-111
104
EDTA plasma (n=5)
95-107
102
Cell culture media (n=5)
95-110
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.2
202.69
870.19
104.96
182.76
940.85
Standard deviation
12.11
15.42
90.59
9.56
21.42
68.31
C V (%)
11.51
7.61
10.41
9.11
11.72
7.26
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CCRL1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CCRL1 in samples. No significant cross-reactivity or interference between Mouse CCRL1 and analogues was observed.
