Mouse CD19 (Cluster of differentiation 19) CLIA Kit
The Mouse CD19 (Cluster of Differentiation 19) CLIA Kit is specifically designed for the quantification of CD19 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and consistent results for various research applications.CD19 is a key protein found on B cells, playing a crucial role in their maturation, activation, and function.
It is a valuable biomarker for studying immune responses, autoimmune diseases, and hematologic malignancies. The Mouse CD19 CLIA Kit provides researchers with a reliable tool to analyze CD19 expression levels, aiding in the understanding of B cell biology and disease mechanisms in mouse models.
Product Name:
Mouse CD19 (Cluster of differentiation 19) CLIA Kit
SKU:
MOES00177
Target:
Mouse CD19 (Cluster of differentiation 19)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CD19. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CD19 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CD19, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CD19. You can calculate the concentration of Mouse CD19 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
91-107
95-111
Average (%)
97
98
103
1:4
Range (%)
101-116
99-115
96-113
Average (%)
108
107
104
1:8
Range (%)
95-108
97-115
101-116
Average (%)
101
105
108
1:16
Range (%)
102-115
87-99
89-102
Average (%)
109
92
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-111
103
EDTA plasma (n=5)
91-104
97
Cell culture media (n=5)
94-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.32
288.57
843.49
109.74
276.55
841.25
Standard deviation
10.35
24.12
91.6
8.89
20.38
81.35
C V (%)
10.02
8.36
10.86
8.1
7.37
9.67
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CD19 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CD19 in samples. No significant cross-reactivity or interference between Mouse CD19 and analogues was observed.
