The Mouse CD3 (Cluster of Differentiation 3) CLIA Kit is specifically designed for the quantitative measurement of CD3 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and precision, ensuring accurate and consistent results for various research applications.CD3 is a vital component of the T-cell receptor complex and plays a crucial role in T-cell activation and immune response. Abnormalities in CD3 expression or function have been linked to autoimmune diseases, immunodeficiency disorders, and transplant rejection.
Therefore, this kit serves as a valuable tool for studying immune system function and developing potential therapeutic interventions.With its reliable performance and ease of use, the Mouse CD3 CLIA Kit is the ideal choice for researchers seeking to explore the role of CD3 in immune-related processes and diseases in mouse models.
Product Name:
Mouse CD3 (Cluster of differentiation 3) CLIA Kit
SKU:
MOES00175
Target:
Mouse CD3 (Cluster of differentiation 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CD3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CD3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CD3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CD3. You can calculate the concentration of Mouse CD3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
95-109
103-119
Average (%)
98
102
109
1:4
Range (%)
89-101
88-102
101-115
Average (%)
96
95
109
1:8
Range (%)
87-100
87-100
87-103
Average (%)
94
93
94
1:16
Range (%)
90-102
84-97
89-103
Average (%)
96
91
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-114
104
EDTA plasma (n=5)
93-110
101
Cell culture media (n=5)
90-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
260.28
787.0
2444.36
256.98
857.65
2403.13
Standard deviation
32.35
59.81
189.93
32.3
67.93
201.14
C V (%)
12.43
7.6
7.77
12.57
7.92
8.37
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CD3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CD3 in samples. No significant cross-reactivity or interference between Mouse CD3 and analogues was observed.
