Mouse CD30L (Cluster of Differentiation 30 Ligand) CLIA Kit
The Mouse CD30L (Cluster of Differentiation 30 Ligand) CLIA Kit is specifically designed for the quantitative detection of mouse CD30L levels in various biological samples, including serum, plasma, and tissue lysates. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for a wide range of research applications.CD30L is a key molecule involved in immune responses and inflammation, playing a critical role in T-cell activation and proliferation.
Dysregulation of CD30L expression has been linked to various autoimmune diseases, allergic reactions, and lymphomas, highlighting its importance as a potential therapeutic target and diagnostic marker.With its easy-to-use format and robust performance, the Mouse CD30L CLIA Kit is an essential tool for researchers studying immune system function, inflammatory disorders, and cancer immunotherapy. Get precise and consistent data with this reliable assay kit from Assay Genie.
Product Name:
Mouse CD30L (Cluster of Differentiation 30 Ligand) CLIA Kit
SKU:
MOES00178
Target:
Mouse CD30L (Cluster of Differentiation 30 Ligand)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CD30L. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CD30L and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CD30L, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CD30L. You can calculate the concentration of Mouse CD30L in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-115
88-103
88-98
Average (%)
106
95
93
1:4
Range (%)
92-106
89-105
88-98
Average (%)
99
96
93
1:8
Range (%)
99-117
92-103
93-108
Average (%)
107
97
99
1:16
Range (%)
90-103
98-113
99-114
Average (%)
95
106
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
101
EDTA plasma (n=5)
101-113
106
Cell culture media (n=5)
89-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.53
271.07
919.67
97.08
297.39
991.44
Standard deviation
11.49
26.97
64.65
12.52
28.1
82.88
C V (%)
12.15
9.95
7.03
12.9
9.45
8.36
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CD30L concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CD30L in samples. No significant cross-reactivity or interference between Mouse CD30L and analogues was observed.
