The Mouse CD45.2 Monoclonal Antibody (PE-conjugated, 104-2) from Assay Genie (Product Code: AGEL1526) is a specialized tool for researchers working with mouse models in the field of immunology. This antibody specifically targets the CD45.2 protein, a key marker for leukocytes and important for immune cell signaling and function.Developed for use in flow cytometry, the PE-conjugated CD45.2 antibody allows for highly sensitive detection and quantification of CD45.2-expressing cells in complex samples. Its monoclonal nature ensures batch-to-batch consistency and high specificity, making it a reliable choice for experiments requiring precise identification and analysis of immune cell populations.
The CD45.2 Monoclonal Antibody is ideal for investigating immune cell subpopulations, tracking cell migration, and studying immune responses in mouse models. Its use can aid in understanding immune system dynamics, evaluating immune cell activation, and exploring immunological processes in various disease contexts.For researchers seeking a dependable tool for flow cytometry-based studies in mouse immunology, the CD45.2 Monoclonal Antibody from Assay Genie provides a valuable resource for advancing research and uncovering insights into immune cell biology.
Product Title:
PE Anti-Mouse CD45.2 Antibody [104.2]
SKU:
AGEL1526
Size:
25µg, 100µg
Clone No:
104.2
Synonyms:
Ly-5.2; LCA;
Host Species:
Mouse
Reactivity:
Mouse
Application:
FCM
Isotype:
Mouse IgG2a, κ
Isotype Control:
PE Mouse IgG2a, κ Isotype Control[C1.18.4]
Form:
Liquid
Conjugation:
PE
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:
Gene ID:
19264
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
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