The Mouse CEA (Carcinoembryonic Antigen) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of CEA levels in mouse serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it a valuable tool for researchers studying cancer and related diseases.CEA is a glycoprotein that is overexpressed in a variety of cancers, including colorectal, pancreatic, and lung cancer. Monitoring CEA levels can help in the early detection, prognosis, and monitoring of cancer progression.
Additionally, CEA levels can be used to evaluate the response to therapy and anticipate recurrence.By using the Mouse CEA CLIA Kit, researchers can gain valuable insights into the role of CEA in cancer development and progression, leading to the development of new diagnostic and therapeutic strategies. This kit is suitable for a wide range of research applications and can provide valuable information for advancing our understanding of cancer biology.
Product Name:
Mouse CEA (Carcinoembryonic Antign) CLIA Kit
SKU:
MOES00145
Target:
Mouse CEA (Carcinoembryonic Antign)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CEA/CD66. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CEA/CD66 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CEA/CD66, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CEA/CD66. You can calculate the concentration of Mouse CEA/CD66 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-111
92-107
98-115
Average (%)
105
100
105
1:4
Range (%)
87-98
92-107
90-103
Average (%)
93
98
97
1:8
Range (%)
100-115
102-117
93-107
Average (%)
106
108
98
1:16
Range (%)
89-102
87-103
101-115
Average (%)
95
94
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-107
98
EDTA plasma (n=5)
86-101
92
Cell culture media (n=5)
100-111
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.38
61.04
171.06
27.43
58.72
173.12
Standard deviation
2.96
6.4
16.05
2.23
6.71
10.59
C V (%)
11.66
10.48
9.38
8.13
11.43
6.12
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CEA/CD66 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CEA/CD66 in samples. No significant cross-reactivity or interference between Mouse CEA/CD66 and analogues was observed.
