The Mouse CFL1 (Cofilin-1, Non-Muscle) CLIA Kit is a reliable tool for accurately measuring levels of Cofilin-1 in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Cofilin-1 is a key protein involved in regulating actin dynamics and cell migration, making it essential for understanding cellular processes such as cytoskeletal organization and cell motility.
Dysregulation of Cofilin-1 has been linked to various diseases including cancer, neurodegenerative disorders, and cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing potential treatments.Overall, the Mouse CFL1 CLIA Kit provides researchers with a valuable tool for investigating the role of Cofilin-1 in various biological processes and diseases, leading to a better understanding of its implications in health and disease.
Product Name:
Mouse CFL1 (Cofilin 1, Non-Muscle) CLIA Kit
SKU:
MOES00187
Target:
Mouse CFL1 (Cofilin 1, Non-Muscle)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CFL1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CFL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CFL1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CFL1. You can calculate the concentration of Mouse CFL1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-118
96-109
86-101
Average (%)
109
103
93
1:4
Range (%)
93-105
85-99
89-103
Average (%)
98
90
95
1:8
Range (%)
102-117
92-104
87-99
Average (%)
108
97
93
1:16
Range (%)
103-116
101-115
90-104
Average (%)
109
109
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
96
EDTA plasma (n=5)
95-112
102
Cell culture media (n=5)
90-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
182.65
434.19
1446.15
190.22
435.27
1455.36
Standard deviation
23.14
37.25
146.93
17.2
50.27
133.6
C V (%)
12.67
8.58
10.16
9.04
11.55
9.18
Sample type &Sample volume:
Tissue homogenates; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CFL1 concentrations in Tissue homogenates.
Specificity:
This kit recognizes Mouse CFL1 in samples. No significant cross-reactivity or interference between Mouse CFL1 and analogues was observed.
