Mouse CGRP (Calcitonin Gene Related Peptide) CLIA Kit
The Mouse CGRP (Calcitonin Gene-Related Peptide) CLIA Kit is a reliable tool for the quantitative measurement of CGRP levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.As a key neuropeptide involved in pain transmission and modulation, CGRP plays a crucial role in various physiological and pathological processes.
Its dysregulation has been implicated in conditions such as migraine, inflammation, and neurogenic inflammation, making it a valuable biomarker for investigating these disorders and developing targeted therapies.Overall, the Mouse CGRP CLIA Kit from Assay Genie provides researchers with a powerful tool for studying the role of CGRP in health and disease, ultimately advancing our understanding of neurobiology and therapeutic interventions.
Product Name:
Mouse CGRP (Calcitonin Gene Related Peptide) CLIA Kit
SKU:
MOES00135
Target:
Mouse CGRP (Calcitonin Gene Related Peptide)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CGRP1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CGRP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CGRP1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CGRP1. You can calculate the concentration of Mouse CGRP1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-115
92-107
97-113
Average (%)
108
98
104
1:4
Range (%)
83-95
97-108
85-96
Average (%)
90
103
91
1:8
Range (%)
95-112
95-110
88-101
Average (%)
103
102
93
1:16
Range (%)
100-118
85-99
84-99
Average (%)
108
92
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
91
EDTA plasma (n=5)
94-110
101
Cell culture media (n=5)
94-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.3
44.41
192.68
22.09
48.44
194.49
Standard deviation
2.17
3.47
16.9
2.21
4.78
18.11
C V (%)
9.31
7.81
8.77
10.0
9.87
9.31
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CGRP1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CGRP1 in samples. No significant cross-reactivity or interference between Mouse CGRP1 and analogues was observed.
