Mouse CGRP (Calcitonin Gene Related Peptide) ELISA Kit
The Mouse CGRP (Calcitonin Gene-Related Peptide) ELISA Kit is specifically designed for the accurate measurement of CGRP levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research purposes.CGRP is a key neuropeptide involved in modulating pain transmission, inflammation, and cardiovascular functions. It plays a crucial role in conditions such as migraine, neurogenic inflammation, and cardiovascular diseases, making it a valuable biomarker for studying these disorders and exploring potential therapeutic interventions.
With its advanced technology and reliable performance, the Mouse CGRP ELISA Kit is an indispensable tool for researchers investigating the role of CGRP in various physiological and pathological processes. Trust this kit to deliver accurate and reproducible data to advance your research endeavors.
Product Name:
Mouse CGRP (Calcitonin Gene Related Peptide) ELISA Kit
SKU:
MOES00791
Target:
Mouse CGRP (Calcitonin Gene Related Peptide)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CGRP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CGRP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CGRP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CGRP1. You can calculate the concentration of Mouse CGRP1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
98-111
91-101
Average (%)
93
105
96
1:4
Range (%)
89-102
82-95
81-95
Average (%)
95
88
87
1:8
Range (%)
93-106
84-95
88-100
Average (%)
99
89
93
1:16
Range (%)
91-104
82-94
81-96
Average (%)
98
89
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
99
EDTA plasma (n=5)
93-104
98
Cell culture media (n=5)
89-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.6
91.7
452.77
49.79
93.25
428.03
Standard deviation
3.33
5.27
22.19
3.28
4.94
14.51
C V (%)
6.45
5.75
4.9
6.59
5.3
3.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse CGRP1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CGRP1 in samples. No significant cross-reactivity or interference between Mouse CGRP1 and analogues was observed.
