The Mouse CHRM2 (Cholinergic Receptor Muscarinic 2) CLIA Kit is specifically designed for the precise measurement of CHRM2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.CHRM2 is a key receptor involved in cholinergic signaling, playing a crucial role in various physiological processes including neurotransmission, muscle contraction, and memory formation.
Dysregulation of CHRM2 has been implicated in conditions such as Alzheimer's disease, schizophrenia, and mood disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Mouse CHRM2 CLIA Kit is a valuable tool for researchers looking to investigate the role of CHRM2 in health and disease, providing reliable and precise measurements that can advance our understanding of cholinergic signaling pathways.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CHRM2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CHRM2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CHRM2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CHRM2. You can calculate the concentration of Mouse CHRM2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-96
95-111
104-117
Average (%)
91
102
110
1:4
Range (%)
99-114
85-97
87-99
Average (%)
107
90
94
1:8
Range (%)
101-116
104-120
97-109
Average (%)
109
110
103
1:16
Range (%)
98-110
96-110
100-111
Average (%)
105
104
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-106
100
EDTA plasma (n=5)
90-104
96
Cell culture media (n=5)
97-112
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.69
213.9
839.12
108.47
198.03
766.22
Standard deviation
12.7
18.63
79.63
11.83
23.29
73.56
C V (%)
12.87
8.71
9.49
10.91
11.76
9.6
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CHRM2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CHRM2 in samples. No significant cross-reactivity or interference between Mouse CHRM2 and analogues was observed.
