Mouse Chromosome 1 Open Reading Frame 85 (C1orf85) ELISA Kit
The Mouse C1orf85 (Chromosome 1 Open Reading Frame 85) ELISA Kit is specially designed for the precise quantification of C1orf85 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results, making it an ideal tool for various research applications.C1orf85 is a protein that plays a crucial role in cellular processes and has been implicated in various biological functions. It is associated with several diseases and disorders, making it a valuable target for studying disease mechanisms and potential therapeutic interventions.
With the Mouse C1orf85 ELISA Kit, researchers can easily and efficiently measure C1orf85 levels in biological samples, enabling detailed analysis and further understanding of its role in health and disease. This kit provides a reliable and user-friendly solution for investigating the functions and implications of C1orf85, contributing to advancements in scientific research and clinical studies.
Product Name:
Mouse Chromosome 1 Open Reading Frame 85 (C1orf85) ELISA Kit
Product Code:
MODL00177
Size:
96 Assays
Target:
C1orf85
Synonyms:
GLMP, Glycosylated Lysosomal Membrane Protein, NCU-G1, Lysosomal protein NCU-G1
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
84-102
93
EDTA plasma (n=5)
91-101
96
Heparin plasma (n=5)
92-98
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
