The Mouse CK-MB (Creatine Kinase-MB Isoenzyme) ELISA Kit is specifically designed for the accurate measurement of CK-MB levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research purposes.Creatine Kinase-MB is an important enzyme in the heart that is released into the bloodstream following heart muscle damage, making it a valuable biomarker for detecting myocardial infarction and other cardiac-related conditions in mice.
The Mouse CK-MB ELISA Kit provides researchers with a reliable tool for studying these conditions and developing potential therapeutic interventions.Overall, the Mouse CK-MB ELISA Kit from AssayGenie is a valuable resource for researchers working in the field of cardiovascular research and preclinical drug development in mouse models.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CKMB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CKMB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CKMB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CKMB. You can calculate the concentration of Mouse CKMB in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
90-101
83-96
Average (%)
98
95
90
1:4
Range (%)
89-101
83-96
90-102
Average (%)
94
89
95
1:8
Range (%)
88-103
86-98
84-98
Average (%)
95
91
89
1:16
Range (%)
90-102
81-93
87-98
Average (%)
95
87
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
100
EDTA plasma (n=5)
94-109
100
Cell culture media (n=5)
88-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
230.22
624.67
2095.16
252.79
661.59
2221.8
Standard deviation
11.74
36.42
75.64
16.91
37.64
99.31
C V (%)
5.1
5.83
3.61
6.69
5.69
4.47
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse CKMB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CKMB in samples. No significant cross-reactivity or interference between Mouse CKMB and analogues was observed.
