Mouse CLCF1 (Cardiotrophin Like Cytokine Factor 1) CLIA Kit
The Mouse CLCF1 (Cardiotrophin-Like Cytokine Factor 1) CLIA kit is specifically designed for the precise quantification of CLCF1 levels in mouse serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.CLCF1 is a crucial cytokine involved in various biological processes, including immune responses, inflammation, and tissue repair.
Its dysregulation has been linked to conditions such as autoimmune diseases, cancer, and metabolic disorders, making it a valuable biomarker for investigating these diseases and potential therapeutic interventions.With its user-friendly protocol and reliable performance, the Mouse CLCF1 CLIA kit is an indispensable tool for researchers studying the role of CLCF1 in health and disease. Order now and accelerate your research with confidence.
Product Name:
Mouse CLCF1 (Cardiotrophin Like Cytokine Factor 1) CLIA Kit
SKU:
MOES00148
Target:
Mouse CLCF1 (Cardiotrophin Like Cytokine Factor 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CLCF1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CLCF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CLCF1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CLCF1. You can calculate the concentration of Mouse CLCF1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-98
89-105
101-114
Average (%)
90
96
106
1:4
Range (%)
89-103
101-116
104-119
Average (%)
96
107
110
1:8
Range (%)
98-111
92-107
89-102
Average (%)
105
99
96
1:16
Range (%)
100-115
99-115
93-107
Average (%)
107
105
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-96
90
EDTA plasma (n=5)
95-110
101
Cell culture media (n=5)
92-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
37.96
91.98
314.32
36.85
100.09
309.91
Standard deviation
3.15
10.49
31.65
4.39
7.49
23.31
C V (%)
8.3
11.4
10.07
11.91
7.48
7.52
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CLCF1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CLCF1 in samples. No significant cross-reactivity or interference between Mouse CLCF1 and analogues was observed.
