Based on the information provided at www.assaygenie.com/mouse-clu-clusterin-clia-kit-moes00180/, the Mouse CLU (Clusterin) CLIA Kit is a cutting-edge assay designed for the accurate measurement of clusterin levels in mouse samples, including serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results to support a variety of research applications.Clusterin, also known as apolipoprotein J, is a multifunctional protein involved in various physiological processes, such as lipid metabolism, apoptosis, and tissue remodeling.
It has been implicated in the pathogenesis of several diseases, including Alzheimer's disease, cancer, and cardiovascular disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.In summary, the Mouse CLU (Clusterin) CLIA Kit from Assay Genie provides researchers with a reliable tool for studying the role of clusterin in health and disease, offering accurate and reproducible results to support their scientific investigations.
Product Name:
Mouse CLU (Clusterin) CLIA Kit
SKU:
MOES00180
Target:
Mouse CLU (Clusterin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CLU. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CLU and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CLU, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CLU. You can calculate the concentration of Mouse CLU in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
94-107
90-102
Average (%)
99
101
95
1:4
Range (%)
93-107
95-111
99-114
Average (%)
98
101
106
1:8
Range (%)
85-98
89-100
98-112
Average (%)
92
94
104
1:16
Range (%)
86-100
91-103
92-106
Average (%)
91
96
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-115
107
EDTA plasma (n=5)
86-100
92
Cell culture media (n=5)
102-116
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.0
5.65
16.25
1.8
5.97
17.67
Standard deviation
0.22
0.62
1.87
0.16
0.64
1.87
C V (%)
11.0
10.97
11.51
8.89
10.72
10.58
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CLU concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CLU in samples. No significant cross-reactivity or interference between Mouse CLU and analogues was observed.
