The Mouse CLU (Clusterin) ELISA Kit is specifically designed for the precise and quantitative measurement of Clusterin levels in various biological samples. Clusterin, a versatile glycoprotein with diverse roles in cellular and physiological processes, is recognized for its involvement in numerous functions, including cell survival, apoptosis, lipid transportation, and regulation of the immune response. Accurate measurement of Clusterin is essential for gaining insights into its multifaceted functions within the body, particularly its implications in disease pathogenesis, neurodegenerative disorders, cancer progression, and renal dysfunction among other conditions.
As a critical player in maintaining cellular homeostasis and responding to various stressors, assessing Clusterin levels provides valuable information for understanding its roles in health and disease. The Mouse CLU ELISA Kit by Assay Genie offers exceptional sensitivity and specificity, ensuring dependable and reproducible results. Manufactured under strict quality control guidelines, this kit guarantees robust performance and ease of use, making it an ideal choice for researchers investigating the intricate functions of Clusterin in biological systems.
Product Name:
Mouse CLU (Clusterin) ELISA Kit
SKU:
AEES00259
Target:
Mouse CLU (Clusterin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 ng/mL
Detection range:
15.63-1000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CLU. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CLU and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CLU, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CLU. You can calculate the concentration of Mouse CLU in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
86-98
91-103
Average (%)
92
91
97
1:4
Range (%)
84-93
86-99
90-105
Average (%)
88
93
96
1:8
Range (%)
86-98
80-92
93-104
Average (%)
93
86
98
1:16
Range (%)
85-96
86-97
91-106
Average (%)
91
92
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
98
EDTA plasma (n=5)
85-98
90
Cell culture media (n=5)
88-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.44
139.9
495.38
45.48
147.22
493.11
Standard deviation
2.99
5.79
22.74
2.92
8.27
15.53
C V (%)
5.92
4.14
4.59
6.43
5.62
3.15
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse CLU concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CLU in samples. No significant cross-reactivity or interference between Mouse CLU and analogues was observed.
