The Mouse CNTF (Ciliary Neurotrophic Factor) CLIA Kit is designed to accurately measure CNTF levels in mouse serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, providing researchers with reliable and reproducible results for various research applications.CNTF is a vital neurotrophic factor that plays a key role in promoting the survival and differentiation of neuronal cells. It is involved in various physiological processes, including neuronal development, repair, and protection.
Dysregulation of CNTF has been associated with neurodegenerative diseases, making it an important biomarker for studying these conditions and potentially developing therapeutic interventions.With this Mouse CNTF CLIA Kit, researchers can explore the role of CNTF in neurological disorders and gain valuable insights into potential treatment strategies. Stay ahead in your research with this advanced kit from Assay Genie.
Product Name:
Mouse CNTF (Ciliary Neurotrophic Factor) CLIA Kit
SKU:
MOES00173
Target:
Mouse CNTF (Ciliary Neurotrophic Factor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CNTF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CNTF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CNTF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CNTF. You can calculate the concentration of Mouse CNTF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
95-112
100-116
Average (%)
103
103
106
1:4
Range (%)
102-118
89-99
97-113
Average (%)
110
94
104
1:8
Range (%)
98-114
85-97
93-108
Average (%)
105
90
100
1:16
Range (%)
88-103
99-115
87-99
Average (%)
95
105
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
97
EDTA plasma (n=5)
96-112
103
Cell culture media (n=5)
97-108
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
43.84
99.64
320.26
40.53
101.45
341.54
Standard deviation
4.18
9.42
32.19
3.45
10.87
21.82
C V (%)
9.53
9.45
10.05
8.51
10.71
6.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CNTF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CNTF in samples. No significant cross-reactivity or interference between Mouse CNTF and analogues was observed.
