The Mouse Complement Component 5 (C5) ELISA Kit is specifically designed for the quantitative measurement of complement component 5 levels in mouse serum, plasma, and cell culture supernatants. This kit offers unparalleled sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.Complement component 5 is a key protein in the complement system, playing a vital role in the body's immune response by triggering inflammation and cell lysis. Abnormal levels of C5 have been implicated in various inflammatory diseases, autoimmune disorders, and infections, making it a valuable biomarker for studying these conditions and potentially developing therapeutic interventions.
With its precise detection capabilities and ease of use, the Mouse C5 ELISA Kit is an indispensable tool for researchers looking to explore the role of complement component 5 in immune-mediated diseases and develop novel treatment strategies. Visit the link provided for more information on ordering this kit.
Product Name:
Mouse Complement Component 5 (C5) ELISA Kit
Product Code:
MODL00183
Size:
96 Assays
Target:
C5
Synonyms:
CPAMD4, C5a anaphylatoxin, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
