The Mouse CP (Ceruloplasmin) ELISA Kit is specifically designed for the precise measurement of ceruloplasmin levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an excellent choice for a variety of research applications.Ceruloplasmin is a vital protein involved in regulating copper levels in the body and has been implicated in various diseases, including Wilson's disease and Alzheimer's disease.
By measuring ceruloplasmin levels, researchers can gain valuable insights into these conditions and potentially develop new therapies. Get your Mouse CP (Ceruloplasmin) ELISA Kit today and unlock the potential for groundbreaking discoveries in your research.
Product Name:
Mouse CP (Ceruloplasmin) ELISA Kit
SKU:
MOES00831
Target:
Mouse CP (Ceruloplasmin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CP. You can calculate the concentration of Mouse CP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
90-103
90-105
Average (%)
98
95
97
1:4
Range (%)
88-99
86-99
84-99
Average (%)
93
91
91
1:8
Range (%)
90-105
81-94
83-94
Average (%)
96
86
89
1:16
Range (%)
85-97
84-97
84-97
Average (%)
92
91
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
83-96
90
EDTA plasma (n=5)
90-102
97
Cell culture media (n=5)
91-109
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.33
17.77
74.93
8.44
16.13
69.37
Standard deviation
0.52
1.04
2.5
0.44
0.75
2.16
C V (%)
5.57
5.85
3.34
5.21
4.65
3.11
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse CP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CP in samples. No significant cross-reactivity or interference between Mouse CP and analogues was observed.
