The Mouse cPLA2 (Phospholipase A2, Cytosolic) CLIA Kit is specifically designed for the accurate detection of cPLA2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing reliable and reproducible results for a variety of research applications.cPLA2 is an important enzyme involved in the regulation of lipid metabolism and inflammatory responses. It plays a critical role in mediating the release of arachidonic acid, which serves as a precursor for various lipid mediators involved in inflammation and immune responses. Dysregulation of cPLA2 activity has been implicated in several diseases, including arthritis, asthma, and cardiovascular disorders, making it a valuable target for therapeutic development and disease research.
The Mouse cPLA2 CLIA Kit is an essential tool for studying the role of cPLA2 in various physiological and pathological processes, providing researchers with a reliable and accurate method for quantifying cPLA2 levels in mouse samples. With its high performance and versatility, this kit is a valuable addition to any laboratory conducting research in the fields of lipid metabolism, inflammation, and immune response.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse cPLA2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse cPLA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse cPLA2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse cPLA2. You can calculate the concentration of Mouse cPLA2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-99
90-103
92-107
Average (%)
91
96
99
1:4
Range (%)
95-108
101-116
95-111
Average (%)
101
109
103
1:8
Range (%)
86-100
95-111
100-116
Average (%)
92
102
107
1:16
Range (%)
91-106
90-103
92-106
Average (%)
97
97
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
93
EDTA plasma (n=5)
98-113
106
Cell culture media (n=5)
97-114
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.39
238.21
829.58
107.72
246.78
823.3
Standard deviation
12.41
22.73
55.5
11.35
26.01
68.09
C V (%)
11.56
9.54
6.69
10.54
10.54
8.27
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse cPLA2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse cPLA2 in samples. No significant cross-reactivity or interference between Mouse cPLA2 and analogues was observed.
