The Mouse CRT (Calreticulin) CLIA Kit is a cutting-edge assay designed for the precise measurement of calreticulin levels in mouse samples. This kit offers exceptional sensitivity and specificity, providing accurate and consistent results for a variety of research purposes.Calreticulin is a key protein involved in several cellular functions, including calcium homeostasis, chaperone activity, and immune responses. Dysregulation of calreticulin has been implicated in various diseases, such as cancer, autoimmune disorders, and inflammation, making it a valuable biomarker for studying these conditions and exploring therapeutic interventions.
With its user-friendly format and robust performance, the Mouse CRT CLIA Kit is an essential tool for researchers investigating the role of calreticulin in health and disease in mouse models. Discover new insights and advancements in your research with this innovative assay.
Product Name:
Mouse CRT (Calreticulin) CLIA Kit
SKU:
MOES00139
Target:
Mouse CRT (Calreticulin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CRT. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CRT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CRT, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CRT. You can calculate the concentration of Mouse CRT in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
101-115
85-99
Average (%)
105
109
92
1:4
Range (%)
100-115
88-102
90-102
Average (%)
106
94
96
1:8
Range (%)
101-117
84-99
93-107
Average (%)
107
90
101
1:16
Range (%)
89-102
93-108
98-115
Average (%)
96
99
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-108
102
EDTA plasma (n=5)
98-113
105
Cell culture media (n=5)
99-114
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.86
222.32
703.65
101.22
212.1
706.4
Standard deviation
13.21
25.19
72.76
13.1
22.16
72.76
C V (%)
12.6
11.33
10.34
12.94
10.45
10.3
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CRT concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CRT in samples. No significant cross-reactivity or interference between Mouse CRT and analogues was observed.
