The Mouse CTGF (Connective Tissue Growth Factor) CLIA Kit is specifically designed for the precise measurement of CTGF levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it perfect for various research purposes.CTGF is a key protein that plays a vital role in connective tissue growth and repair.
It is involved in processes such as fibrosis, wound healing, and tissue remodeling, making it a crucial biomarker for studying these conditions and potentially developing therapeutic interventions.Overall, the Mouse CTGF CLIA Kit is a valuable tool for researchers looking to investigate the role of CTGF in various physiological and pathological processes in mouse models.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CTGF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CTGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CTGF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CTGF. You can calculate the concentration of Mouse CTGF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-115
97-110
99-112
Average (%)
107
104
104
1:4
Range (%)
91-102
102-118
98-112
Average (%)
97
109
104
1:8
Range (%)
95-108
100-117
93-105
Average (%)
101
107
98
1:16
Range (%)
99-114
86-101
86-100
Average (%)
106
93
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-110
104
EDTA plasma (n=5)
86-100
93
Cell culture media (n=5)
87-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.13
316.59
841.86
100.52
310.84
821.86
Standard deviation
12.01
22.64
92.27
9.85
28.6
51.28
C V (%)
12.12
7.15
10.96
9.8
9.2
6.24
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CTGF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CTGF in samples. No significant cross-reactivity or interference between Mouse CTGF and analogues was observed.
