The Mouse CTGF (Connective Tissue Growth Factor) ELISA Kit is specifically designed for the accurate measurement of CTGF levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing researchers with reliable and reproducible results for a variety of research applications.CTGF is an important protein that plays a key role in regulating cell growth, differentiation, and tissue repair. It is involved in the development of connective tissues and is implicated in various diseases such as fibrosis, arthritis, and cancer.
Understanding CTGF levels can provide valuable insights for studying these conditions and developing potential therapeutic interventions.Overall, the Mouse CTGF ELISA Kit is a valuable tool for researchers looking to investigate the role of CTGF in health and disease, providing precise measurements and accurate results for their studies.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CTGF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CTGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CTGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CTGF. You can calculate the concentration of Mouse CTGF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
94-107
94-111
Average (%)
101
99
102
1:4
Range (%)
86-100
86-100
85-99
Average (%)
92
91
91
1:8
Range (%)
89-102
84-98
85-95
Average (%)
94
91
90
1:16
Range (%)
87-98
85-99
88-101
Average (%)
93
90
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
90
EDTA plasma (n=5)
93-104
99
Cell culture media (n=5)
86-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.47
1.48
3.93
0.48
1.61
4.17
Standard deviation
0.03
0.07
0.13
0.03
0.07
0.21
C V (%)
6.38
4.73
3.31
6.25
4.35
5.04
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse CTGF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CTGF in samples. No significant cross-reactivity or interference between Mouse CTGF and analogues was observed.
