The Mouse CTLA4 (Cytotoxic T-Lymphocyte-Associated Antigen 4) CLIA Kit is a powerful tool for accurately measuring levels of CTLA4 in mouse samples, including serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring reliable and consistent results for a variety of research applications.CTLA4 is a critical protein involved in regulating the immune response by inhibiting T cell activation, making it a key player in autoimmune diseases, inflammation, and cancer.
By accurately measuring CTLA4 levels, researchers can gain valuable insights into immune system function and potential therapeutic targets.Overall, the Mouse CTLA4 CLIA Kit is a valuable resource for researchers studying immune regulation, autoimmune diseases, and cancer, providing the tools needed to further understand the role of CTLA4 in these conditions and potentially develop new treatment strategies.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CTLA4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CTLA4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CTLA4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CTLA4. You can calculate the concentration of Mouse CTLA4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
95-112
84-97
Average (%)
101
102
91
1:4
Range (%)
96-111
92-108
102-117
Average (%)
104
99
108
1:8
Range (%)
98-112
89-100
94-108
Average (%)
104
94
100
1:16
Range (%)
98-115
95-106
98-113
Average (%)
106
100
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
98
EDTA plasma (n=5)
97-113
105
Cell culture media (n=5)
101-118
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.19
275.02
739.19
121.2
301.85
670.49
Standard deviation
13.52
29.65
57.88
9.91
33.14
57.39
C V (%)
12.27
10.78
7.83
8.18
10.98
8.56
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CTLA4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CTLA4 in samples. No significant cross-reactivity or interference between Mouse CTLA4 and analogues was observed.
