The Mouse CTSD (Cathepsin D) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of Cathepsin D levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for a variety of research applications.Cathepsin D is a lysosomal protease that plays a critical role in protein degradation and processing. Abnormal levels of Cathepsin D have been associated with various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases.
Studying Cathepsin D levels can provide valuable insights into disease mechanisms and potential therapeutic interventions.The Mouse CTSD CLIA Kit offers researchers a powerful tool for investigating the role of Cathepsin D in disease pathology and for developing novel therapeutic strategies. Its high sensitivity and specificity make it an indispensable tool for cutting-edge research in the field of molecular biology and biomedicine.
Product Name:
Mouse CTSD (Cathepsin D) CLIA Kit
SKU:
MOES00158
Target:
Mouse CTSD (Cathepsin D)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CTSD. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CTSD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CTSD, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CTSD. You can calculate the concentration of Mouse CTSD in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-119
95-112
92-104
Average (%)
109
103
98
1:4
Range (%)
97-114
95-111
94-105
Average (%)
104
102
100
1:8
Range (%)
88-102
97-112
89-101
Average (%)
95
104
95
1:16
Range (%)
92-104
95-109
101-115
Average (%)
97
103
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
98
EDTA plasma (n=5)
99-111
105
Cell culture media (n=5)
91-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
36.84
91.12
332.3
39.59
93.08
351.84
Standard deviation
4.03
9.25
32.23
3.85
7.95
36.27
C V (%)
10.94
10.15
9.7
9.72
8.54
10.31
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CTSD concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CTSD in samples. No significant cross-reactivity or interference between Mouse CTSD and analogues was observed.
