The Mouse CTSG (Cathepsin G) ELISA Kit is a powerful tool for measuring cathepsin G levels in mouse serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable asset for various research studies.Cathepsin G is a key enzyme involved in immune responses, inflammation, and tissue remodeling. Its dysregulation has been linked to various diseases, including autoimmune disorders and cardiovascular conditions.
By measuring cathepsin G levels, researchers can gain insights into disease mechanisms and potential therapeutic targets.This ELISA kit provides a user-friendly and efficient method for quantifying cathepsin G levels, enabling researchers to explore its role in disease pathology and therapeutic interventions effectively. Trust in the Mouse CTSG ELISA Kit for reliable and precise measurements in your research endeavors.
Product Name:
Mouse CTSG (Cathepsin G) ELISA Kit
SKU:
MOES01827
Target:
Mouse CTSG (Cathepsin G)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CTSG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CTSG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CTSG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CTSG. You can calculate the concentration of Mouse CTSG in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-111
94-111
85-96
Average (%)
104
101
90
1:4
Range (%)
90-105
86-98
88-98
Average (%)
97
91
93
1:8
Range (%)
93-105
86-101
87-100
Average (%)
99
92
93
1:16
Range (%)
88-100
85-98
81-95
Average (%)
93
90
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-104
98
EDTA plasma (n=5)
87-98
92
Cell culture media (n=5)
91-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.77
116.9
416.24
53.14
116.01
443.15
Standard deviation
3.2
5.24
18.73
2.92
4.71
24.15
C V (%)
5.84
4.48
4.5
5.49
4.06
5.45
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse CTSG concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CTSG in samples. No significant cross-reactivity or interference between Mouse CTSG and analogues was observed.
