Mouse CTX I (Cross Linked C-telopeptide of Type I Collagen) ELISA Kit
The Mouse CTXⅠ (Cross-Linked C-Telopeptide of Type Ⅰ Collagen) ELISA Kit is a specialized assay designed for the precise and quantitative measurement of CTXⅠ levels in mouse samples.
CTXⅠ is a valuable biomarker widely used in bone metabolism studies, especially for assessing bone resorption and turnover processes in various physiological and pathological conditions. Our ELISA kit offers exceptional sensitivity and specificity, enabling accurate and reproducible results crucial for researchers investigating bone health, osteoporosis, and related disorders. Manufactured under stringent quality control standards, this kit provides reliable performance and ease of use, making it an excellent choice for studies focused on understanding bone remodeling dynamics and assessing bone health.
Product Name:
Mouse CTX I (Cross Linked C-telopeptide of Type I Collagen) ELISA Kit
SKU:
AEES00218
Target:
Mouse CTXⅠ (Cross Linked C-telopeptide of Type Ⅰ Collagen)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CTXⅠ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CTXⅠ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CTXⅠ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CTXⅠ. You can calculate the concentration of Mouse CTXⅠ in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
88-99
93-105
Average (%)
96
93
100
1:4
Range (%)
91-100
86-101
94-104
Average (%)
97
94
98
1:8
Range (%)
87-100
100-108
102-108
Average (%)
95
105
105
1:16
Range (%)
95-108
92-100
92-107
Average (%)
103
97
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
91
EDTA plasma (n=5)
86-96
91
Cell culture media (n=5)
94-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
82.17
221.85
948.92
87.03
203.37
803.81
Standard deviation
4.48
10.85
48.3
4.99
9.56
33.28
C V (%)
5.45
4.89
5.09
5.73
4.7
4.14
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse CTXⅠ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CTXⅠ in samples. No significant cross-reactivity or interference between Mouse CTXⅠ and analogues was observed.
