Mouse CTXI (Cross Linked C-telopeptide of Type I Collagen) CLIA Kit
The Mouse CTXI (Cross-linked C-telopeptide of Type I Collagen) CLIA Kit is specifically designed for the precise measurement of CTXI levels in mouse serum, plasma, and tissue culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.Cross-linked C-telopeptide of Type I Collagen is a marker of bone resorption and degradation, making it a valuable tool for studying bone metabolism and turnover.
Elevated levels of CTXI are associated with conditions such as osteoporosis, osteoarthritis, and other bone-related disorders, making this kit essential for researchers investigating these areas and developing potential therapeutic interventions.Get reliable and robust data with the Mouse CTXI CLIA Kit, allowing for in-depth analysis and insights into bone health and disease progression in mouse models.
Product Name:
Mouse CTXI (Cross Linked C-telopeptide of Type I Collagen) CLIA Kit
SKU:
MOES00206
Target:
Mouse CTXI (Cross Linked C-telopeptide of Type I Collagen)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CTXI. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CTXI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CTXI, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CTXI. You can calculate the concentration of Mouse CTXI in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-112
95-108
95-108
Average (%)
105
102
103
1:4
Range (%)
88-102
85-96
100-115
Average (%)
94
91
107
1:8
Range (%)
89-103
88-103
92-105
Average (%)
97
95
97
1:16
Range (%)
89-103
97-113
88-102
Average (%)
94
105
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-117
108
EDTA plasma (n=5)
89-101
96
Cell culture media (n=5)
89-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.44
221.09
767.41
106.23
217.59
839.54
Standard deviation
8.49
18.77
73.44
13.43
19.19
55.91
C V (%)
8.71
8.49
9.57
12.64
8.82
6.66
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CTXI concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CTXI in samples. No significant cross-reactivity or interference between Mouse CTXI and analogues was observed.
