The Mouse CX3CL1 (Chemokine C-X3-C motif ligand 1) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of CX3CL1 levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for a variety of research applications.CX3CL1 is a chemokine involved in immune response regulation and cell recruitment, playing a key role in inflammatory processes and immune system functioning. Dysregulation of CX3CL1 has been implicated in various diseases, including autoimmune disorders, neurodegenerative conditions, and cardiovascular diseases, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
Overall, the Mouse CX3CL1 CLIA Kit is a valuable tool for researchers looking to investigate the role of CX3CL1 in health and disease, offering precise and accurate measurement of CX3CL1 levels in biological samples.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CX3CL1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CX3CL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CX3CL1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CX3CL1. You can calculate the concentration of Mouse CX3CL1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
98-113
89-105
Average (%)
93
104
96
1:4
Range (%)
98-112
86-98
90-101
Average (%)
105
93
95
1:8
Range (%)
94-108
94-109
98-115
Average (%)
99
101
106
1:16
Range (%)
93-109
102-116
101-115
Average (%)
100
108
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
96
EDTA plasma (n=5)
101-113
107
Cell culture media (n=5)
100-113
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
213.14
626.42
1730.09
213.41
668.86
1829.02
Standard deviation
27.28
44.29
198.44
20.91
55.65
165.71
C V (%)
12.8
7.07
11.47
9.8
8.32
9.06
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CX3CL1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CX3CL1 in samples. No significant cross-reactivity or interference between Mouse CX3CL1 and analogues was observed.
