The Mouse CX3CL1 (Chemokine C-X3-C Motif Ligand 1) ELISA Kit is specifically designed for the precise measurement of CX3CL1 levels in mouse serum, plasma, and tissue lysates. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an invaluable tool for a wide range of research applications.CX3CL1 is a chemokine protein involved in inflammation and immune response, playing a crucial role in the communication between immune cells and endothelial cells.
Dysregulation of CX3CL1 has been implicated in various inflammatory diseases, making it a key biomarker for investigating these conditions and potential therapeutic interventions.With the Mouse CX3CL1 ELISA Kit, researchers can confidently study the role of CX3CL1 in inflammation, immune response, and disease pathology, paving the way for advancements in our understanding of these complex processes.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CX3CL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CX3CL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CX3CL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CX3CL1. You can calculate the concentration of Mouse CX3CL1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
89-104
99-112
Average (%)
97
95
104
1:4
Range (%)
94-109
80-90
85-100
Average (%)
99
85
91
1:8
Range (%)
94-109
84-95
82-97
Average (%)
100
89
89
1:16
Range (%)
90-102
84-97
83-94
Average (%)
96
89
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-112
102
EDTA plasma (n=5)
94-108
99
Cell culture media (n=5)
92-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.01
2.25
7.58
0.94
2.2
7.13
Standard deviation
0.06
0.11
0.32
0.06
0.12
0.24
C V (%)
5.94
4.89
4.22
6.38
5.45
3.37
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse CX3CL1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CX3CL1 in samples. No significant cross-reactivity or interference between Mouse CX3CL1 and analogues was observed.
