The Mouse CX3CR1 (Chemokine C-X3-C Motif Receptor 1) CLIA Kit is specifically designed for the accurate measurement of CX3CR1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.CX3CR1 is a key receptor involved in mediating immune responses and inflammation, playing a vital role in immune cell recruitment and migration.
Dysfunction of CX3CR1 has been implicated in various diseases, including autoimmune disorders, inflammatory conditions, and neurodegenerative diseases. The Mouse CX3CR1 CLIA Kit provides researchers with a valuable tool for studying the role of CX3CR1 in these diseases and exploring potential therapeutic interventions.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CX3XR1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CX3XR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CX3XR1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CX3XR1. You can calculate the concentration of Mouse CX3XR1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-104
101-117
87-99
Average (%)
99
108
94
1:4
Range (%)
99-113
86-100
86-98
Average (%)
106
93
92
1:8
Range (%)
94-109
98-114
88-103
Average (%)
102
106
95
1:16
Range (%)
100-114
92-106
89-101
Average (%)
107
99
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-116
107
EDTA plasma (n=5)
100-114
106
Cell culture media (n=5)
91-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.73
281.37
670.12
118.9
261.35
662.4
Standard deviation
10.85
21.19
62.12
12.5
23.31
59.81
C V (%)
9.98
7.53
9.27
10.51
8.92
9.03
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CX3XR1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CX3XR1 in samples. No significant cross-reactivity or interference between Mouse CX3XR1 and analogues was observed.
