The Mouse CX43 (Connexin-43) CLIA Kit is a highly reliable and sensitive assay designed for the detection of Connexin-43 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high specificity and accuracy, ensuring precise and consistent results for a variety of research applications.Connexin-43 is a key protein involved in cell-to-cell communication and gap junction formation, playing a crucial role in various physiological processes such as cardiac function, neuronal signaling, and tissue development. Dysregulation of Connexin-43 has been implicated in a range of diseases, including cardiovascular disorders, neurodegenerative conditions, and certain cancers, making it a valuable biomarker for studying these pathologies and exploring potential therapeutic interventions.
Overall, the Mouse CX43 (Connexin-43) CLIA Kit provides researchers with a powerful tool for investigating the role of Connexin-43 in health and disease, offering reliable and accurate measurements to advance our understanding of cellular communication and tissue homeostasis in mouse models.
Product Name:
Mouse CX43 (Connexin 43) CLIA Kit
SKU:
MOES00198
Target:
Mouse CX43 (Connexin 43)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CX43. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CX43 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CX43, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CX43. You can calculate the concentration of Mouse CX43 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-121
88-100
100-113
Average (%)
110
93
106
1:4
Range (%)
86-99
93-107
88-102
Average (%)
92
100
95
1:8
Range (%)
85-100
86-100
97-111
Average (%)
91
93
105
1:16
Range (%)
101-115
99-111
89-101
Average (%)
108
105
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-112
103
EDTA plasma (n=5)
99-116
108
Cell culture media (n=5)
85-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.62
242.93
784.24
99.07
231.77
711.1
Standard deviation
11.67
19.7
54.19
10.67
18.33
58.59
C V (%)
11.83
8.11
6.91
10.77
7.91
8.24
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CX43 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CX43 in samples. No significant cross-reactivity or interference between Mouse CX43 and analogues was observed.
