The Mouse CXCL15 (Chemokine C-X-C motif ligand 15) ELISA Kit is a highly reliable and accurate tool designed for the detection of CXCL15 levels in mouse serum, plasma, and cell culture supernatants. This kit boasts excellent sensitivity and specificity, ensuring consistent and precise results for a variety of research purposes.CXCL15 is an important chemokine involved in immune response regulation, inflammation, and cell recruitment. It plays a critical role in various physiological and pathological processes, including cancer, inflammatory diseases, and immune disorders.
As such, the Mouse CXCL15 ELISA Kit is a valuable resource for studying these conditions and exploring potential therapeutic interventions.Overall, the Mouse CXCL15 ELISA Kit is a powerful tool for researchers looking to investigate the role of CXCL15 in disease pathogenesis and develop novel treatment strategies.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CXCL15. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CXCL15 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CXCL15, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CXCL15. You can calculate the concentration of Mouse CXCL15 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-108
84-97
91-101
Average (%)
102
91
96
1:4
Range (%)
88-100
86-98
84-99
Average (%)
94
91
90
1:8
Range (%)
87-99
86-97
86-99
Average (%)
93
92
91
1:16
Range (%)
90-104
84-97
81-94
Average (%)
97
89
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-107
98
EDTA plasma (n=5)
93-109
100
Cell culture media (n=5)
87-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
236.71
548.76
2092.72
259.34
580.06
1969.19
Standard deviation
14.2
25.57
91.66
15.33
29.29
72.07
C V (%)
6
4.66
4.38
5.91
5.05
3.66
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse CXCL15 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CXCL15 in samples. No significant cross-reactivity or interference between Mouse CXCL15 and analogues was observed.
