The Mouse CXCL16 (Chemokine C-X-C motif ligand 16) CLIA Kit is specifically designed for precise measurement of CXCL16 levels in mouse serum, plasma, and tissue homogenates. With its exceptional sensitivity and specificity, this kit delivers reliable and consistent results, making it an invaluable tool for a variety of research applications.CXCL16 is a critical chemokine involved in the recruitment of immune cells and regulation of inflammation. Its dysregulation has been linked to various inflammatory conditions, autoimmune diseases, and cancer progression.
By accurately quantifying CXCL16 levels, researchers can gain insights into the underlying mechanisms of these diseases and design potential therapeutic strategies.Overall, the Mouse CXCL16 CLIA Kit provides researchers with a powerful tool to study the role of CXCL16 in health and disease, paving the way for innovative discoveries and advancements in the field of immunology and inflammation research.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CXCL16. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CXCL16 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CXCL16, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CXCL16. You can calculate the concentration of Mouse CXCL16 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-111
84-100
94-106
Average (%)
102
91
100
1:4
Range (%)
86-102
98-112
103-118
Average (%)
93
104
109
1:8
Range (%)
87-99
86-97
86-99
Average (%)
93
91
92
1:16
Range (%)
85-98
84-97
84-98
Average (%)
90
90
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-110
100
EDTA plasma (n=5)
99-117
107
Cell culture media (n=5)
87-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
38.46
103.45
302.15
40.76
103.39
327.95
Standard deviation
4.98
8.62
22.69
4.68
11.17
21.78
C V (%)
12.95
8.33
7.51
11.48
10.8
6.64
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CXCL16 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CXCL16 in samples. No significant cross-reactivity or interference between Mouse CXCL16 and analogues was observed.
