The Mouse CYB561 (Cytochrome B-561) CLIA Kit is a powerful tool for the accurate quantification of Cytochrome B-561 levels in mouse serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, providing researchers with precise and consistent results for a variety of experimental purposes.Cytochrome B-561 is a key protein involved in oxidative reactions and electron transport processes within cells.
It plays a critical role in maintaining cellular homeostasis and has been implicated in various diseases, making it a valuable target for research and drug development.With the Mouse CYB561 CLIA Kit, researchers can explore the role of Cytochrome B-561 in physiological and pathological processes, paving the way for a better understanding of cellular function and potential therapeutic interventions.
Product Name:
Mouse CYB561 (Cytochrome b-561) CLIA Kit
SKU:
MOES00217
Target:
Mouse CYB561 (Cytochrome b-561)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CYB561. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CYB561 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CYB561, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CYB561. You can calculate the concentration of Mouse CYB561 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-101
93-105
99-112
Average (%)
92
98
106
1:4
Range (%)
94-112
100-115
103-118
Average (%)
102
106
110
1:8
Range (%)
98-111
89-100
99-115
Average (%)
105
94
107
1:16
Range (%)
101-117
91-105
95-106
Average (%)
107
97
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
94
EDTA plasma (n=5)
89-103
95
Cell culture media (n=5)
96-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.97
2.47
9.11
0.91
2.42
8.89
Standard deviation
0.11
0.24
0.89
0.11
0.22
0.84
C V (%)
11.34
9.72
9.77
12.09
9.09
9.45
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CYB561 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CYB561 in samples. No significant cross-reactivity or interference between Mouse CYB561 and analogues was observed.
